Abstract—This paper introduces VPUF Physical OS, the world’s first hardware-native trust substrate designed to establish deterministic biophysical phase coherence for secure wearables and Artificial General Intelligence (AGI) humanoid safety. Traditional Physical Unclonable Functions (PUFs) suffer from environmental instability and failure in genetic proximitydiscrimination[cite: 155, 283]. We propose a biophysical kernel that binds MEMS phase entropy to Ballistocardiogram (BCG) anchors using a Lyapunov-stable operator[cite: 5, 156, 284]. Wefurther establish stochastic stability using Itô calculus to prove noise-resilient quilibrium [cite: 157, 285]. Experimental results on unmodified COTS hardware (iPhone 11 Pro) demonstrate a terminal stability of 0.0072 rad and a 24.7s lock-on time[cite: 6, 158, 286]. Validation with 9 genetically related samplesconfirms an inter-subject correlation of -0.0093[cite: 7, 159, 287]. Furthermore, a Monte Carlo simulation (N=1,000) yields a near-ideal Hamming Distance (HD=0.4731), establishing VPUF as a post-cryptographic standard for DTx and humanoid safety[cite: 160, 288].Index Terms—PUF, Hardware Security, DTx, Lyapunov Stability, Stochastic Differential Equations, Genetic Proximity, Humanoid Safety, AGI Alignment[cite: 8, 161, 289].