This study examined the effects of 12 biochemical agents, including chemotherapies, a dilution series of Etoposide, copper (II) ions, and other bioactive compounds, on tumor cell migration using a fibroblast scratch assay. Agents were applied to adult mouse fibroblasts, and migration was quantified via microscopy and cell counts. Chemotherapies such as 5-Fluorouracil, Cytarabine, Etoposide, and Cycloheximide showed varied effects based on mechanisms like DNA synthesis inhibition and apoptosis induction. Copper (II) ions and Vanadate promoted mitochondrial damage and tumor suppressor activation, while Retinoic Acid inhibited proliferation and metastasis-related signaling. Resveratrol demonstrated the strongest inhibition of cell migration, with logistic growth modeling suggesting lasting effects on tumor proliferation. These results highlight how Resveratrol can act as a dual-action therapeutic and support further investigation into its use in cancer treatment.