Physics of Biology

    

Yeast-Based Expression of Zika Virus Envelope Protein and CTB Fusion for Oral Vaccine Development

Authors: Hyunho Shin

This study describes the development of an expression system for an oral Zika virus vaccine. The vaccine is based on a fusion protein of the cholera toxin B subunit (CTB) and the Zika virus envelope protein (E protein), connected via a GGSG linker and expressed in Saccharomyces cerevisiae. The ADH1 promoter was incorporated into the yeast expression vector to drive transcription of the fusion protein. Yeast transformants were confirmed through colony PCR, and initial protein expression was validated using SDS-PAGE.

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[v1] 2024-11-21 22:07:22

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