Physics of Biology

    

Modification of Campylobacter Cytolethal Distending Toxin for Selective Targeting of PD-L1 Expressing Cancer Cells

Authors: Hyunho Shin

Cytolethal Distending Toxin (CDT), a tripartite exotoxin from Campylobacter jejuni, is known for inducing DNA damage and apoptosis. In this study, we engineered the A subunit (CDTA) to selectively target cancer cells by replacing its native binding region with anti-PD-L1 antibody sequences. Using sequence data from UniProt (CDTA: A1VXG4, PD-L1: Q9NZQ7), we identified and modified CDTA’s residues 129-140. Structural validation using AlphaFold 2 confirmed that these modifications retained CDTA’s structural stability and affinity for PD-L1, supporting its potential as a targeted cancer therapeutic. Future experimental steps include protein expression, purification, and cytotoxicity testing to confirm the toxin’s selective binding and apoptotic effects on PD-L1 positive cancer cells.

Comments: 8 Pages.

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[v1] 2024-11-06 21:59:08

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