With the world recording abnormally high temperatures in September, 2023, it has been analyzed that the possibility of the collapse of the Maginot Line of climate change, which scholars are concerned about, has increased.[1] Increased temperatures may have adverse effects on their production performance and animal welfare.[2] This study was conducted using wild-type Escherichia Coli(DH5-α) which is not resistant to antibiotics. The Escherichia Coli(DH5-α) was grown in a liquid medium and later mixed with a saline solution containing 0.8% NaCl. After dilution, 10µl of the solution was taken with a micropipette and spread evenly on an LB agar plate. The plate was then incubated at 37°C for 10 hours. Afterwards, the resulting colony was transferred to the LB broth medium using an inoculation loop. There were a total of 3 groups: the control group, experimental group 1, and experimental group 2. The control group was cultured at 37°C for 24 hours, while experimental group 1 was heat-treated at 42°C for 15 minutes, and experimental group 2 was heat-treated at 42°C for 30 minutes. Each group was subcultured to the third stage.