Biochemistry

    

Anomalous Datasets Reveal Metagenomic Fabrication Pipeline That Further Questions the Legitimacy of Ratg13 Genome and the Associated Nature Paper

Authors: John F. Signus

Recently, Daoyu Zhang et. Al [1], Mona Ralker et. Al [2] and Mohit Singla et. al [3] have reported exceptional anomalies associated with the RaTG13 metagenomic dataset which was inconsistent with that of a real fecal sample. Despite extensive search, we are only able to isolate 2 datasets Other than RaTG13 itself, that possessed significant levels of non-adaptor repeat sequences and absence of bacteria in the context of “bat” and “fecal” or “virome”. Furthermore, the analysis of such datasets have revealed an established pipeline of which a viral sequence is “rehosted”—e.g. added to a metagenomic sample that originally did not contain such viral sequences. This raises serious questions to the legitimacy of RaTG13 genome and the associated Nature paper.

Comments: 37 Pages. Multiple alignment to all known viral genomes (at a sensitivity higher than NCBI analysis) made possible using the SERRATUS toolbox.

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Submission history

[v1] 2020-10-21 08:53:45

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