Raoultella ornithinolytica is belongs to the family of Enterobacteriaceae, a Gram-negative encapsulated aerobic bacillus associated with bacteremia and urinary tract infections. As biofield therapy is increasingly popular in biomedical heath care, so present study aimed to evaluate the impact of Mr. Trivedi’s biofield treatment on antimicrobial sensitivity, minimum inhibitory concentration (MIC), biochemical study, and biotype number of multidrug resistant strain of R. ornithinolytica. Clinical sample of R. ornithinolytica was divided into two groups i.e. control and biofield treated which were analyzed for the above parameters using MicroScan Walk-Away® system on day 10 after treatment. Antimicrobial sensitivity assay results showed a significant increase (60.71%) in sensitivity pattern of antimicrobials i.e. changed from resistant to susceptible while 10.71% of tested antimicrobials changed from intermediate to susceptible as compared to control. MIC results showed a significant decrease in MIC values of 71.88% tested antimicrobials as compared to control. Biochemical reaction study showed 15.15% alteration in different biochemical such as cetrimide, cephalothin, kanamycin, and ornithine after biofield treatment as compared to control. A significant change in biotype number (7775 4370) was also observed with organism identified as Klebsiella oxytoca after biofield treatment as compared to control (7775 5372). Overall results conclude that biofield treatment could be used as complementary and alternative treatment strategy against multidrug resistant strain of R. ornithinolytica with respect to improve the sensitivity and reduce the MIC values of antimicrobials. Hence, it is assumed that biofield treatment might be a suitable cost effective treatment strategy in near future, which could have therapeutic value in patients suffering from multidrug resistant pathogens.